Together these results showed that hTERT‐immortalized CMCs retained major characteristics of mammary epithelial cells, and stability of the genome is required for maintaining normal mammary epithelium function. Furthermore, αs1‐ and β‐casein gene was induced in luminal CMCs in response to lacto‐hormones stimulation. In terms of functional differentiation, luminal CMCs organized into alveolus‐like structures when grown in Matrigel. CMCs with abnormal karyotypes lost p53 protein after chemical‐induced DNA damage and showed anchorage‐independent growth in soft agar assay. Karyotypic analysis showed three CMCs retained their modal Capra hircus chromosome number (2n = 60), whereas the remaining two CMCs were abnormal at 2n = 19 and 2n = 36. Telomeric repeat amplification protocol revealed various telomerase activities in CMCs associated with their distinct proliferation potential. Five immortalized CMCs were assigned to either myoepithelial or luminal epithelial groups based on their morphology and expression of cell lineage‐specific intermediate filaments. Ke, MW Hsu, JT Jiang, YN Cheng, WTK Ju, YTĬontents The aim of this article is to demonstrate and characterize caprine mammary epithelial cells (CMC) immortalized with human telomerase reverse transcriptase (hTERT) gene. Characterization of hTERT‐Immortalized Caprine Mammary Epithelial Cells Characterization of hTERT‐Immortalized Caprine Mammary Epithelial Cells
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